[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-4-9] [發(fā)布人: ] [閱讀次數(shù):] [返回]

HiCrome™ Salmonella Chromogen Agar
(Salmonella Chromogen Agar, HiCrome™)

(Rambach Equivalent Agar)

培養(yǎng)基配方

Composition per liter:
Agar ............................................................................................15.0g
Peptic digest of animal tissue........................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Meat extract ..................................................................................1.0g
Na-deoxycholate...........................................................................1.0g
Chromogenic mixture.................................................................1 vial
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Mix to completely dissolve components. Do not autoclave. Mix and boil in 5 min sequences for 35–40 min. Cool to 50°C. Shake gently for 30–35 min. Pour into sterile Petri dishes.
Use: A selective medium used for the detectgion of Salmonella species. This medium exploits a novel phenotypic characteristic of Salmonella spp.: the formation of acid from propylene glycol. This characteristic may be used in combination with a chromogenic indicator of β-galactosidase to differentiate Salmonella spp. from Proteus spp. and the other members of the Enterobacteriaceae. Deoxycholate is included in the plate medium as an inhibitor of Gram-positive organisms. Nontyphi Salmonella spp. yield distinct, bright red colonies on this medium, allowing facilitated identification and unambiguous differentiation from Proteus spp. Coliforms produce blue-green to blue-violet colonies. Other Enterobacteriaceae and Gram-negative bacteria such as Proteus, Shigella, Pseudomonas, Salmonella typhi, and S. paratyphi A form colorless or yellow colonies.