[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2021-4-9] [發(fā)布人: ] [閱讀次數(shù):] [返回]

HiCrome™ Salmonella Agar

(Salmonella Agar, HiCrome™)

培養(yǎng)基配方

Composition per liter:
Agar ............................................................................................13.0g
Peptic digest of animal tissue........................................................6.0g
Chromogenic mixture...................................................................5.4g
Yeast extract..................................................................................2.5g
Chromogenic mix .........................................................................1.5g
Bile salt mixture............................................................................1.0g
pH 7.7 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Mix to completely dissolve components. Do not autoclave. Cool to 50°C. Pour into sterile Petri dishes.
Use: A selective chromogenic medium used for the isolation and differentiation of Salmonella species from coliforms in food and water. E. coli and Salmonella are easily distinguishable due to the colony characteristics. Salmonella give light purple colonies with a halo. E. coli have a characteristic blue color. Other organisms give colorless colonies. The characteristic light purple and blue color is due to the chro- mogenic mixture. Chromogenic medium for detecting and identifying Enterobacteria, Proteus species, and other Gram-positive organisms.