[所屬分類:培養(yǎng)基配方] [發(fā)布時(shí)間:2021-4-9] [發(fā)布人: ] [閱讀次數(shù):] [返回]

HiCrome™ OGYE Agar Base
(OGYE Agar Base HiCrome)
(Oxytetracycline Glucose

Yeast Extract Agar HiCrome)

培養(yǎng)基配方

Composition per liter:
Glucose ......................................................................................20.0g
Agar............................................................................................12.0g
Yeast extract .................................................................................4.0g
Chromogenic mixture ..................................................................1.1g
Oxytetracycline selective supplement .....................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Oxytetracycline Selective Supplement:
Composition per 10.0mL :
Oxytetracycline.............................................................................0.1g
Preparation of Oxytetracycline Selective Supplement: Add 0.1g oxytetracycline to 10.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except oxytetracycline selective supplement, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL sterile oxytetracycline selective supplement. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the rapid isolation of yeasts and molds from milk and milk products. Oxytetracycline makes the medium more selective by inhibiting the growth of lactobacilli. Aspergillus niger appears as light blue colored colonies with black spores due to the presence of chromogenic mixture; Candida albicans shows green colored colonies and Saccharomyces cerevisiae gives colorless colonies.