[所屬分類:培養(yǎng)基配方] [發(fā)布時間:2020-6-29] [發(fā)布人:] [閱讀次數:] [返回]

山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dubos Salts Medium
(DSMZ Medium 1161)

培養(yǎng)基配方
Composition per liter:
Agar........................................................................15.0g
K2HPO4 ......................................................................1.0g
MgSO4 ·7H2O.................................................................0.5g
KCl..........................................................................0.5g
NaNO3 .......................................................................0.5g
FeSO4 ·7H2O...............................................................10.0mg
Filter paper.............................................................Variable
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except filter paper, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 7.0. Gently heat while stirring and bring to boiling. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes. For tubes allow
to solidify on a slant. When the agar has solidified, place a strip of ster-
ile filter paper on to the surface of each slope. Inoculate on to the filter
paper.
Use: For the cultivation of Cellvibrio spp.